Transpiration will NOT be offered as an live lab demonstration for Hinton 2021.
Big Ideas & AP Biology Science Practices
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Transpiration Student Experimental Protocol
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TechBoston Academy - Thursday, December 19, 2024
Tosteson Medical Education Building Lab Room 132 3:00 - 5:30 PM
Lab Assistant: Maria Amadeo 
Kennedy Academy of Health Careers & Boston English High School - Thursday, January 9, 2025
Tosteson Medical Education Building Learning Lab Rooms 102 and 132, 3:00 - 5:30 PM
Lab Assistants : Chris Etienne 
Jennifer Su
Boston Latin Academy - Tuesday, January 14, 2025
Tosteson Medical Education Building Learning Lab Room 332, 3:00 - 5:30PM
Lab Assistant: Jennifer Su
Boston Latin Academy - Thursday, January 16,2025
Tosteson Medical Education Building Learning Lab Rooms 102 and 132, 3:00 - 5:30PM
Lab Assistants: Alan Wong
Wilaysha Evens 
Transpiration Student Experimental Protocol
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| LabQuest Data Collection (5.1) | Manual Data Collection (5.2) |
Manual Recording Data Form
Students will manually record data using this handout.
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Plant Specimens
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| 1 - |
| Syngonium | 2 - |
| Fasiata | 3 - |
| Begonia Rex | 4 - |
| Ctenanthe Setosa | 5 - |
| Philodendron Cordatum - common name: pothos |
Creating a leaf impression for microscopy
PDFview-file name handout_exolabs_microscopy_handout_v4v6.pdf height 400
Materials:
Microscope Slide
Leaf (identify the leaf so you may accurately label your microphotograph)
Clear Tape
Nail Polish
Microscope Slide
Microscope with EXO Labs Focus Camera App and iPad interface
1. Choose a leaf and identify it from the plants listed above. The stoma of the leaf are predominately on the underside of the leaf so work with the underside of the leaf you have chosen.
2. Coat a section of the underside of the leaf with nail polish. Coat a piece of the tape with nail polish as well. Place the nail polish coated section of the tape over the nail polish coated section of the leaf and put aside to dry (about 10 minutes)
3. When the nail polish is dry, carefully lift the tape with the nail polish that now holds an impression of the leaf carefully up, and place the tape on your microscope slide.
4. Place your slide with the nail polish impression on the microscope stage. Turn the objective lens to 10X.
5. Using the focusing mechanism of the microscope, but viewing the microscope image from the iPad, bring the image of the leaf into focus. The Exo Camera and the iPad require a second to process the image so the focusing process will lag several seconds behind your actual focusing movements. Be patient, allow the image to fully process before you try additional focusing.
6. When you have your leaf impression in focus look for the following leaf structures to label.
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Your instructor will assist you with using the labeling and pointing tools on the iPad. See Digital Microscope ExoLabs Focus Camera handout.
7. Show the scale of the micrograph you have created by using the EXO Camara's calibrated measuring tool. Make sure you use the correct calibration (4x or 10x) before using the scale tool.
8. Label your micrograph below the image:
Title: Title should include plant name and magnification level.
Note: Group member first names and date.
9. Write microscope image(s) names and group name on Microscope Log sheet next to iPad station. We will use this form to download and post digital copies of your images.
10. Enjoy your beautiful micrograph!
