Lab 4 Transpiration

TechBoston Academy - Thursday, December 19, 2024

Tosteson Medical Education Building Lab Room 132   3:00 - 5:30 PM

Lab Assistant: Maria Amadeo

Kennedy Academy of Health Careers & Boston English High School - Thursday, January 9, 2025

Tosteson Medical Education Building Learning Lab Rooms 102  and 132,  3:00 - 5:30 PM  

Lab Assistants : Chris Etienne

      Jennifer Su

Boston Latin Academy - Tuesday, January 14, 2025

Tosteson Medical Education Building Learning Lab Room 332,  3:00 - 5:30PM

Lab Assistant:  Jennifer Su

Boston Latin Academy - Thursday, January 16,2025

Tosteson Medical Education Building Learning Lab Rooms 102 and 132,  3:00 - 5:30PM

Lab Assistants:  Alan Wong

  Wilaysha Evens

Transpiration Student Experimental Protocol

Manual Recording Data Form

Students will manually record data using this handout.

Plant Specimens 

Open	Open	Open	Open	Open
1 - Syngonium	2 - Fasiata	3 - Begonia Rex	4 - Ctenanthe Setosa	5 - Philodendron Cordatum - common name: pothos

Creating a leaf impression for microscopy

Materials:
Microscope Slide
Leaf (identify the leaf so you may accurately label your microphotograph)
Clear Tape
Nail Polish
Microscope Slide
Microscope with EXO Labs Focus Camera App and iPad interface

1. Choose a leaf and identify it from the plants listed above. The stoma of the leaf are predominately on the underside of the leaf so work with the underside of the leaf you have chosen.

2. Coat a section of the underside of the leaf with nail polish. Coat a piece of the tape with nail polish as well. Place the nail polish coated section of the tape over the nail polish coated section of the leaf and put aside to dry (about 10 minutes)

3. When the nail polish is dry, carefully lift the tape with the nail polish that now holds an impression of the leaf carefully up, and place the tape on your microscope slide.

4. Place your slide with the nail polish impression on the microscope stage. Turn the objective lens to 10X.

5. Using the focusing mechanism of the microscope, but viewing the microscope image from the iPad, bring the image of the leaf into focus. The Exo Camera and the iPad require a second to process the image so the focusing process will lag several seconds behind your actual focusing movements. Be patient, allow the image to fully process before you try additional focusing.

6. When you have your leaf impression in focus look for the following leaf structures to label.