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To edit the htseq bash script:

Code Block
nano htseq.sh

To test the pipeline run the following command if you did alignment by bwa as on this page. If you did alignment using bowtie2 following this page, please change -p bwaOut to -p bowtieOut, or if you used star following this page, please change -p bwaOut to -p starOut. Jobs will not be submitted to the scheduler.

Code Block
runAsPipeline "htseq.sh -p bwaOut -r dm3" "sbatch -p short --mem 4G -t 101:0:0 -n 1" useTmp

To run the pipeline:

Code Block
runAsPipeline "htseq.sh -p bwaOut -r dm3" "sbatch -p short --mem 4G -t 101:0:0 -n 1" useTmp run

To understand how 'runAsPipeline' works, please visit: Run Bash Script As Slurm Pipeline through rcbio/1.2