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cp /home/ld32/rcbioDev/bin/rsemBowtie2.sh .

Now you can modify the options as needed. Please reference the http://deweylab.github.io/RSEM/ if you have any questions.

To edit the Bowtie2 and RSEM bash script:

Code Block
nano rsemBowtie2.sh

To test the pipeline run the following command. Jobs will not be submitted to the scheduler.

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runAsPipeline "rsemBowtie2.sh -r mm10" "sbatch -p short --mem 6G -t 2:0:0 -n 1" useTmp

# this is a test run

To run the pipeline:

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runAsPipeline "rsemBowtie2.sh -r mm10" "sbatch -p short --mem 6G -t 2:0:0 -n 1" useTmp run 2>&1 | tee output.log

# notice here 'run 2>&1 | tee output.log' is added to the command

To understand how 'runAsPipeline' works, please visit: Run Bash Script As Slurm Pipeline through rcbio/1.1